Blue Protein Loading Dye from New England Biolabs

Product Specs
ItemBlue Protein Loading Dye
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberB7703S
Quantity8 ml
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

Specifications/Features

Information:
Blue Loading Buffer Reagents for SDS-PAGE:

3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature)

30X Reducing Agent (1 ml): 1.25 M DTT (Store at -20°C.)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Firing of Replication Origins Is Disturbed by a CDK4/6 Inhibitor in a pRb-Independent MannerInternational Journal of Molecular SciencesJune 25, 2023Su-Jung Kim, Chrystelle Maric, Lina-Marie Briu, Fabien Fauchereau, Giuseppe Baldacci, Michelle Debatisse, Stéphane Koundrioukoff, Jean-Charles Cadoretof cells for each condition. The pellets were incubated in Blue Loading Buffer (New England Biolabs, B7703S, New England Biolabs, Ipswich, MA, USA) with 0.1 unit/mL Benzonase (Merck, 70664-3, Merck, Darmstadt,

(2) SARS-CoV-2 Inhibits NRF2-Mediated Antioxidant Responses in Airway Epithelial Cells and in the Lung of a Murine Model of InfectionMicrobiology SpectrumJune 15, 2023Yue Qu, Aline Haas de Mello, Dorothea R. Morris, Yava L. Jones-Hall, Teodora Ivanciuc, Rachel A. Sattler, Slobodan Paessler, Vineet D. Menachery, Roberto P. Garofalo, Antonella Casolaexperiments. Western blotting. Cells were lysed directly in the cell culture plates with 3× SDS loading buffer (B7703S; New England BioLabs, Ipswich, MA, USA). After boiling at 95 to 100°C for 10 min, the samples were removed

(3) Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m(3)C modificationCell stem cellMarch 2, 2023Zhang F, Yoon K, Zhang DY, Kim NS, Ming GL, Song H.Medium,EMSCO/FISHER,Cat#NC9428056 B-27® Supplement,Gibco,Cat#17504044 Blue Protein Loading sample buffer,New England Biolabs,Cat#B7703S CHIR-99021,StemCell Technologies,Cat#72054 Chloramphenicol,Sigma-Aldrich,Cat#C0378 Chloroform,Sigma-Aldrich,Cat#C2432 "cOmplete,

(4) Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genomeCommunications biologyNovember 25, 2022Estève PO, Sen S, Vishnu US, Ruse C, Chin HG, Pradhan S.1% Triton X-100. The beads were mixed with 1× SDS–PAGE sample loading buffer (New England Biolabs # B7703S) and incubated at 98 °C for 5 min. The protein mixtures were separated on a 10% polyacrylamide Tricine

(5) O-GlcNAc transferase is important for homology-directed repairDNA Repair (Amst)November 1, 2022Xiaoli Ping, Jeremy M. Starksonication. For immunoblots, the samples were denatured in 1xSDS sample loading dye (New England Biolabs, #B7703S). For dot blots, the samples were mixed with loading buffer (20% SDS, 1M Tris-HCl (pH 6.8), 100 mM B-mercaptoethanol,

(6) Anthocyanin Encapsulated Nanoparticles as a Pulmonary Delivery SystemOxidative Medicine and Cellular LongevitySeptember 10, 2022Madumani Amararathna, David W. Hoskin, H. P. Vasantha Rupasingheanti-actin antibodies were from Cell Signaling Technology, Danvers, MA, USA. Blue loading buffer (cat. no. B7703S) was purchased from New England BioLabs Inc., Ipswich, MA, USA. The chemiluminescence (ECL) based Clarity™

(7) MTG8 interacts with LHX6 to specify cortical interneuron subtype identityNature communicationsSeptember 5, 2022Asgarian Z, Oliveira MG, Stryjewska A, Maragkos I, Rubin AN, Magno L, Pachnis V, Ghorbani M, Hiebert SW, Denaxa M, et al.X-100). Protein complexes were eluted from the beads with 80 µl 1x Loading dye (New England BioLabs, B7703S) solution at 55 °C for 10 min. Western Blotting Loading dye and DTT (New England BioLabs, #B7703S)

(8) Hydrogen Sulfide Donor GYY4137 Rescues NRF2 Activation in Respiratory Syncytial Virus InfectionAntioxidants (Basel)July 21, 2022Aline Haas Haas de Mello, Tianshuang Liu, Roberto P. Garofalo, Antonella Casolapromyelocytic leukemia (PML) protein Western blot, the cells were lysed directly with SDS loading buffer (B7703S, New England BioLabs, Ipswich, MA, USA), followed by a brief sonication (10–15 s) to reduce the sample

(9) eNAMPT actions through nucleus accumbens NAD⁺/SIRT1 link increased adiposity with sociability deficits programmed by peripuberty stressScience advancesMarch 4, 2022Morató L, Astori S, Zalachoras I, Rodrigues J, Ghosal S, Huang W, Guillot de Suduiraut I, Grosse J, Zanoletti O, Cao L, et al.standard (Merck, #P5369), and samples were boiled for 5 min in loading buffer (New England Biolabs Inc., #B7703S). Protein homogenates (35 μg) were separated by SDS–polyacrylamide gel electrophoresis (Mini-PROTEAN

(10) DNA damage stress-induced translocation of mutant FUS proteins into cytosolic granules and screening for translocation inhibitorsFrontiers in molecular neuroscienceJanuary 1, 2022Nogami M, Sano O, Adachi-Tominari K, Hayakawa-Yano Y, Furukawa T, Iwata H, Ogi K, Okano H, Yano M.al., 2021b). U251 MG (KO) cells were collected into 1× SDS Blue loading buffer (NEB, Tokyo, Japan; #B7703S) and heated at 95°C for 5 min. Next, genomic DNA in the samples was sheared using a syringe needle (Terumo,

(11) Activation of a signaling pathway by the physical translocation of a chromosomeDevelopmental CellAugust 1, 2021Mathilde Guzzo, Allen G. Sanderlin, Lennice K. Castro, Michael T. LaubRecombinant Proteins","Chemicals, Peptides, and Recombinant Proteins" Blue loading Buffer pack,NEB,Cat #: B7703S Carbenicillin,Goldbio,Cat #: C-103-100 Chloramphenicol,Sigma,Cat #: 60378 dNTP solution mix,NEB,Cat

(12) Hypertrophic cardiomyopathy-linked mutation in troponin T causes myofibrillar disarray and pro-arrhythmic action potential changes in human iPSC cardiomyocytesJournal of Molecular and Cellular CardiologyJanuary 1, 2018Lili Wang, Kyungsoo Kim, Shan Parikh, Adrian Gabriel Cadar, Kevin R. Bersell, Huan He, Jose R. Pinto, Dmytro O. Kryshtal, Bjorn C. Knollmannthe cryopreserved day 30 hiPSC-CMs were lysed with 1X SDS sample buffer (New England BioLabs Inc., #B7703S). 5–10 μL protein samples were loaded onto a 15% SDS-PAGE gel. Digestion was performed with a ProteoExtract All-in-One Trypsin Digestion Kit

(13) BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombinationNature communicationsSeptember 13, 2017Feng W, Jasin M.After extensive washes with 0.05% Tween 20 in PBS, proteins were eluted with SDS-PAGE sample buffer (B7703S, NEB). Western blotting Equal amounts of protein samples (whole-cell lysate, subcellular fractions,

(14) Selectivity and Kinetic Requirements of HDAC Inhibitors as Progranulin Enhancers for Treating Frontotemporal DementiaCell chemical biologyJuly 20, 2017She A, Kurtser I, Reis SA, Hennig K, Lai J, Lang A, Zhao WN, Mazitschek R, Dickerson BC, Herz J, et al.ready for use. Before use, lysates were boiled at 95°C with SDS loading buffer (New England BioLabs #B7703S) + DTT (New England BioLabs #B7705S) for 5 min. In probing for PGRN, proteins were separated on NuPAGE

(15) Nasal neuron PET imaging quantifies neuron generation and degenerationThe Journal of clinical investigationFebruary 1, 2017Van de Bittner GC, Riley MM, Cao L, Ehses J, Herrick SP, Ricq EL, Wey HY, O'Neill MJ, Ahmed Z, Murray TK, et al.each diluted to a concentration of 1.5 μg/μl, mixed with one-third volume of 3× SDS loading buffer (B7703S; NEB), and heated to 95°C for 10 minutes. Ten micrograms of total protein was loaded in each lane and

(16) Elevated free fatty acid uptake via CD36 promotes epithelial-mesenchymal transition in hepatocellular carcinomaScientific reportsOctober 1, 2015Nath A, Li I, Roberts LR, Chan C.protein levels were quantified with micro BCA assay (Pierce, 23235), reduced in Laemmli buffer (NEB, B7703S) and resolved on 10% SDS-PAGE gels by loading 20–40 ug of protein per well. The resolved proteins were

(17) Secondary Structure of Corona Proteins Determines the Cell Surface Receptors Used by NanoparticlesThe Journal of Physical Chemistry BDecember 11, 2014Candace C. Fleischer, Christine K. Payneprotein–NP complex was resuspended in either buffer containing 6% (w/v) SDS (New England Biolabs, #B7703S) or water. Samples were diluted with a 4X Laemmli buffer (Boston Bioproducts, BP-110R) and boiled for

(18) Auto-regulation of the Sohlh1 gene by the SOHLH2/SOHLH1/SP1 complex: implications for early spermatogenesis and oogenesisPloS oneJanuary 1, 2014Shuichi Toyoda, Takuji Yoshimura, Junya Mizuta, Jun-ichi MiyazakiThe extracted protein was mixed 2∶1 v/v with 3x sample buffer (New England BioLabs, Beverly, MA; Cat#B7703S) and a 1/30 volume of 1.25 M dithiothreitol (DTT), after which it was heated at 99°C for 5 min, separated

(19) Cellular binding of anionic nanoparticles is inhibited by serum proteins independent of nanoparticle compositionBiomaterials ScienceSeptember 1, 2013Candace C. Fleischer, Umesh Kumar, Christine K. PayneAu NP pellet was resuspended in a 6% w/v sodium dodecyl sulfate (SDS) buffer (New England Biolabs, B7703S) to remove the protein corona from the surface of the NPs. Supernatants collected after each wash step

(20) Nanoparticle Surface Charge Mediates the Cellular Receptors Used by Protein–Nanoparticle ComplexesThe Journal of Physical Chemistry BAugust 2, 2012Candace C. Fleischer, Christine K. Paynewash. After the final wash, the sample was suspended in buffer containing 6% SDS (New England Biolabs, #B7703S) to remove the protein from the NP surface. Supernatant was diluted by 50% in Laemmli buffer (Boston

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11/21/2019SDS-PAGE Sample Loading Buffer from NEB Hyo AhnConvenient sample loading buffer.